Type I collagen modified titanium sheet improves the proliferation of human adipose-derived mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2014.25.017

Abstract

Abstract:

BACKGROUND: Titanium and titanium alloy are used mostly in artificial joints, fracture fixation, and oral transplantation, while there are complex cases of insufficient bone mass in these areas. The deepened research of stem cells offers a solution for bone injury to promote new bone formation. The biocompatibility of titanium and stem cells and optimization of titanium surface modification have aroused people's attention.
OBJECTIVE: To investigate whether the biocompatibility of titanium and human adipose-derived mesenchymal stem cells can be improved by type I collagen modification of titanium sheets.
METHODS: The experiment was divided into two groups. Modification group: titanium sheet was modified with type I collagen; control group: titanium sheet was not modified with type I collagen. Human adipose-derived mesenchymal stem cells at passage 6 were implanted into titanium sheet in two groups. Then we calculated the number of adherent cells in two groups at 1, 2 and 4 hours after implantation, and compared the cell adhesion rate. MTT assay was used to observe the proliferation of cells on titanium sheet at 2, 4, 6 and 8 days after implantation. DNA and protein content of cells were detected at 3, 6, 9 days after implantation. The growth of human adipose-derived mesenchymal stem cells seeded upon the titanium sheets was observed under scanning electron microscope at 6 days.
RESULTS AND CONCLUSION: When the cells were cultured for 1 hour and 2 hours, the number of adherent cells in the modification group was higher than in the control group (P < 0.05). The absorbance of cells in two groups was increased as the culture time, as detected by MTT assay. The modification group had a significantly higher absorbance value than the control group at 4, 6, 8 days (P < 0.05). DNA and protein contents of the cells in the modification group were higher than that in control group at 6 and 9 days (P < 0.05). At 6 days, the number of adherent cells and secretion of adherent stromal cell matrix in the modification group were significantly better than that in control group, observed by scanning electron microscopy. Type I collagen modified titanium sheets have good surface activity and biocompatibility, and can promote the proliferation of human adipose-derived mesenchymal stem cells.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: titanium, collagen, mesenchymal stem cells, biocompatible materials

CLC Number:

R318

Ye Chuan, Ma Min-xian, Zhang Tao, Tang Jin, Sun Bo, Yang Long, Ren Hou-xiang, Sun Qi, Yang Jing, Zhang Jun-biao. Type I collagen modified titanium sheet improves the proliferation of human adipose-derived mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(25): 4032-4037.

Figures/Tables 1

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